![]() Although no polymorphism has been detected in the coding region of angiopoietin-1, three independent polymorphisms have been identified for angiopoietin-2. Intron-specific primers were used to amplify each exon of angiopoietin-1 and angiopoietin-2 from individual genomic DNAs. The putative RNA transcription start site for each angiopoietin gene was also determined. As would be expected from the homology of these three proteins, the positions of the introns in the three genes are highly conserved. Introns and exons are part of the cells DNA genetic code, but exons encode proteins while introns are non-coding sequences. The exact positions at the intron/exon boundaries and the lengths of all eight introns were determined. Exons code for proteins, whereas introns do not. ![]() In this study the genomic structures of all three human angiopoietins were characterised by direct sequencing of human genomic DNA from the appropriate P1 artificial chromosome (PAC) clones. General introduction to Gene expression in prokaryotes and eukaryotes- Prokaryotic. The mechanism by which they contribute to angiogenesis is thought to involve regulation of endothelial cell interactions with supporting perivascular cells. All angiopoietins bind with similar affinity to the endothelial cell-specific receptor, Tie2. The family of human angiopoietins comprises factors with important roles in vascular development and angiogenesis. Genomic DNA sequences were aligned with cDNA se- quences and with the GenBank database to identify exon/intron boundaries and internal repeats using the. ![]()
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